BD-PE3503-CD5 (4W14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CD5 (4W14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3503

产品名称

CD5 (4W14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CD5

推荐应用

反应种属

Human,Monkey

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

稀释度

WB 1:500~2000, FCM 1:100

预测分子量

54.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation (1). CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling (2).

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CD5 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3503) (1:500)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody (BD-PE3503), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3503) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.