Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded MCF7 (PD-L1 Negative cell line) cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded MDA-MB-231 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded LoVo (PD-L1 Negative cell line) cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded SK-MEL-28 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded NCI-H2228 cell pellets using anti-CD274 (PD-L1) mouse monoclonal antibody (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human Esophagus using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)

Western blot analysis of extracts (35ug) from 5 different cancer cell lines by using anti-CD274 monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-CD274 (PD-L1) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 2.5 min, BD-PE3492) (1:300)