PD-L1 Luminex ELISA with 9E12 Capture (BD-PE3488) and 4D4 Detection Antibodies. Substrate used: Recombinant Human PD-L1

PD-L1 ELISA with 2C7 Capture and 9E12 Detection (BD-PE3488) Antibodies. Substrate used: Recombinant Human PD-L1

PD-L1 ELISA with 9E12 Capture (BD-PE3488) and 4D4 Detection Antibodies. Substrate used: Recombinant Human PD-L1

PD-L1 Luminex ELISA with 2C7 Capture and 9E12 Detection (BD-PE3488) Antibodies. Substrate used: Recombinant Human PD-L1

Immunocytochemistry staining of stable expression CD274 cells using anti-CD274 mouse monoclonal antibody (BD-PE3488) (Left). The right is negative control. (1:5000) (1:2000)

Flow cytometric Analysis of stable expression CD274 in HEK293T cells (Red) compared to negative control HEK293T cells (Blue) using anti-CD274 antibody (BD-PE3488) (1:100).

PD-L1 ELISA with 13G7 Capture and 9E12 Detection (BD-PE3488) Antibodies. Substrate used: Recombinant Human PD-L1

Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (BD-PE3488), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD274 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD274 (1:500). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.