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ACAD9 (17M9) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE3474
产品名称
ACAD9 (17M9) Mouse Monoclonal antibody
类别
常规抗体
基因名称
ACAD9
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human ACAD9 (NP_054768) produced in HEK293T cell.
稀释度
WB 1:500~2000, IHC 1:500
预测分子量
68.6kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes a member of the acyl-CoA dehydrogenase family. Members of this family of proteins localize to the mitochondria and catalyze the rate-limiting step in the beta-oxidation of fatty acyl-CoA. The encoded protein is specifically active toward palmitoyl-CoA and long-chain unsaturated substrates. Mutations in this gene cause acyl-CoA dehydrogenase family member type 9 deficiency. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-ACAD9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3474) (1:500)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ACAD9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3474) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAD9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAD9 (Cat# BD-PE3474)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ACAD9 monoclonal antibody (1:500).

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