BD-PE3438-ANAPC2 (Apc2) (17M11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ANAPC2 (Apc2) (17M11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3438

产品名称

ANAPC2 (Apc2) (17M11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ANAPC2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ANAPC2(NP_037498) produced in HEK293T cell.

稀释度

WB 1:500~2000, IF 1:100

预测分子量

93.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The product of this gene is a component of the complex and shares sequence similarity with a recently identified family of proteins called cullins, which may also be involved in ubiquitin-mediated degradation. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANAPC2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Anti-ANAPC2 mouse monoclonal antibody (BD-PE3438) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ANAPC2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANAPC2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANAPC2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.