BD-PE3377-ANP32C (8J19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ANP32C (8J19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3377

产品名称

ANP32C (8J19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ANP32C

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ANP32C(NP_036535) produced in E.coli.

稀释度

WB 1:2000, IHC 1:2000

预测分子量

26.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Phosphoprotein 32 (PP32) is a tumor suppressor that can inhibit several types of cancers, including prostate and breast cancers. The protein encoded by this gene is one of at least two proteins that are similar in amino acid sequence to PP32 and are part of the same acidic nuclear phosphoprotein gene family. However, unlike PP32, the encoded protein is tumorigenic. The tumor suppressor function of PP32 has been localized to a 25 amino acid region that is divergent between PP32 and the protein encoded by this gene. This gene does not contain introns. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ANP32C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3377) (1:2000)

Flow cytometric Analysis of permeabilized MCF-7 cells, using anti-ANP32C antibody (BD-PE3377, Red), compared to an IgG isotype control (green), and negative control (PBS, Blue) (1:100).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ANP32C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3377) (1:2000)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ANP32C mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3377) (1:2000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANP32C (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANP32C (Cat# BD-PE3377)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.