Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-NONO monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3293)