BD-PE3253-CHEK2 (CHK2) (19N8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CHEK2 (CHK2) (19N8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3253

产品名称

CHEK2 (CHK2) (19N8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Chk2

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CHEK2 (NP_009125) produced in HEK293T cell.

稀释度

WB 1:500~1000, IF 1:50~100, FCM 1:100

预测分子量

60.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition,this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CHEK2 monoclonal antibody.

Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-CHEK2 monoclonal antibody (1:200).

Anti-CHEK2 mouse monoclonal antibody (BD-PE3253) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHEK2 .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CHEK2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

Immunofluorescent staining of HT29 cells using anti-CHEK2 mouse monoclonal antibody (BD-PE3253).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHEK2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHEK2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHEK2 antibody (BD-PE3253), and then analyzed by flow cytometry.