BD-PE3236-ATP5I (11Y9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

ATP5I (11Y9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3236

产品名称

ATP5I (11Y9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

ATP5ME

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human ATP5I (NP_009031) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:500

预测分子量

7.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ATP5I mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3236) (1:500)

Immunohistochemical staining of paraffin-embedded Human appendix tissue within the normal limits using anti-ATP5I mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3236) (1:500)

Immunohistochemical staining of paraffin-embedded Human gastric tissue within the normal limits using anti-ATP5I mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE3236) (1:500)

Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ATP5I monoclonal antibody (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP5I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP5I (Cat# BD-PE3236)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.