Immunofluorescent staining of HT29 cells using anti-BTN3A2 mouse monoclonal antibody (BD-PE3216).

Figure from citation: Vg2Vd2 T cells proliferate in response to the 20.1 but not the 1A6 mAb, although both are specific for BTN3. The 12G12 Vg2Vd2 T cell clone was cultured with control IgG1 mAb, BTN3A2 (1A6) mAb, or 20.1 mAb in the presence of mitomycin C–treated Va2 cells. The cells were pulsed with [3H]thymidine after 24 h and harvested 18 h later.

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)

Anti-BTN3A2 mouse monoclonal antibody (BD-PE3216) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTN3A2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTN3A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTN3A2(Cat# BD-PE3216). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Immunohistochemical staining of paraffin-embedded lung tissue within the normal limits using anti-BTN3A2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3216, Dilution 1:50)