BD-PE3199-SOX5 (13M18) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SOX5 (13M18) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3199

产品名称

SOX5 (13M18) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SOX5

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SOX5(NP_008871) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100

预测分子量

83.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG3

背景介绍

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SOX5 mouse monoclonal antibody (BD-PE3199). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded human kidney using anti-SOX5 mouse monoclonal antibody (BD-PE3199) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Weak cytoplasmic staining is seen in the tubule epithelial cells of the kidney.

Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-SOX5 mouse monoclonal antibody (BD-PE3199) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is seen in the tumor cells.

Immunohistochemical staining of paraffin-embedded human pacreatic cancer using anti-SOX5 mouse monoclonal antibody (BD-PE3199) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong cytoplasmic staining is seen in the tumor cells.

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-SOX5 mouse monoclonal antibody (BD-PE3199) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong nuclear and weak cytoplasmic staining is seen in the tumor cells.

Immunohistochemical staining of paraffin-embedded human skin using anti-SOX5 mouse monoclonal antibody (BD-PE3199) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH 8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Strong nuclear and cytoplasmic staining is seen in the epidermal cells.

Immunohistochemical staining of paraffin-embedded Human testicular cancer tissue using anti-SOX5 mouse monoclonal antibody. (BD-PE3199; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)