BD-PE3175-RIPK3 (13G8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RIPK3 (13G8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3175

产品名称

RIPK3 (13G8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RIPK3

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 233-518 of human RIPK3 (NP_006862) produced in E.coli.

稀释度

WB 1:200 -1:2000, IHC 1:150

预测分子量

56.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The product of this gene is a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, and contains a C-terminal domain unique from other RIP family members. The encoded protein is predominantly localized to the cytoplasm, and can undergo nucleocytoplasmic shuttling dependent on novel nuclear localization and export signals. It is a component of the tumor necrosis factor (TNF) receptor-I signaling complex, and can induce apoptosis and weakly activate the NF-kappaB transcription factor. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-RIPK3 monoclonal antibody at 1:200.

Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3175)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RIPK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3175)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIPK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIPK3.