产品名称
OSTCN Rabbit Polyclonal Antibody
蛋白名称
Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
存储缓冲液
Liquid in PBS containing 50% glycerol, and 0.02% New type preservative N.
Human Swissprot No.
P02818
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P02818/entry
Mouse Swissprot No.
P86546
Mouse Swissprot Link
http://www.uniprot.org/uniprot/P86546
Rat Swissprot Link
http://www.uniprot.org/uniprot/O54941P04640
免疫原
Synthesized peptide derived from part region of human protein AA range: 31-80
特异性
OSTCN Polyclonal Antibody detects endogenous levels of protein.
稀释度
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000 Not yet tested in other applications.
宿主
Polyclonal, Rabbit,IgG
背景介绍
This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015],
组织表达
Brain,Cerebellum,Colon,Lung carcinoma,Skin,Testis,Uterus,
功能
function:Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.,function:Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.,induction:By polyamine analogs in analog-sensitive H157 cells.,online information:Osteocalcin entry,PTM:Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.,similarity:Belongs to the osteocalcin/matrix Gla protein family.,similarity:Contains 1 Gla (gamma-carboxy-glutamate) domain.,subcellular location:Associated with the kinetochore.,subunit:Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Interacts with COPS7A. Interacts via its coiled-coil domain with the leucine-zipper domain of NFE2L2. The interaction with NFE2L2 is required for the transcriptional regulation of SSAT.,tissue specificity:Highest levels of expression in heart and skeletal muscle, with significant levels expressed in kidney and liver.,
纯化
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
