BD-PE3149-METAP2 (Methionine Aminopeptidase 2) (8H10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

METAP2 (Methionine Aminopeptidase 2) (8H10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3149

产品名称

METAP2 (Methionine Aminopeptidase 2) (8H10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

Methionine Aminopeptidase 2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 2-212 of human METAP2 (NP_006829) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

52.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene is a member of the methionyl aminopeptidase family and encodes a protein that binds 2 cobalt or manganese ions. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. Increased expression of this gene is associated with various forms of cancer and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. A pseudogene of this gene is located on chromosome 2. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-METAP2 monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-METAP2 antibody (BD-PE3149), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

Anti-METAP2 mouse monoclonal antibody (BD-PE3149) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY METAP2 .

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-METAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3149)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY METAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-METAP2(Cat# BD-PE3149). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.