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RUVBL2 (Reptin/TIP49B) (5O5) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE3099
产品名称
RUVBL2 (Reptin/TIP49B) (5O5) Mouse Monoclonal antibody
类别
常规抗体
基因名称
RUVBL2
推荐应用
WB
反应种属
Human,Dog,Rat,Monkey,Mouse
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Human recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in HEK293T cell.
稀释度
WB 1:500~2000, IHC 1:150
预测分子量
51kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3099)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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