BD-PE3098-RUVBL2 (Reptin/TIP49B) (6P7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

RUVBL2 (Reptin/TIP49B) (6P7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3098

产品名称

RUVBL2 (Reptin/TIP49B) (6P7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

RUVBL2

推荐应用

反应种属

Human,Dog,Rat,Monkey,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragmengt corresponding to amino acids 113-370 of human RUVBL2(NP_006657) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

51kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3098)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RUVBL2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3098)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RUVBL2 antibody (BD-PE3098), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3098)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3098)

Anti-RUVBL2 mouse monoclonal antibody (BD-PE3098) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2 .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-RUVBL2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-RUVBL2 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2(Cat# BD-PE3098). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.