BD-PE3095-PDE10A (8D17) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PDE10A (8D17) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3095

产品名称

PDE10A (8D17) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PDE10A

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full-length protein expressed in 293T cell transfected with human PDE10A expression vector

稀释度

WB 1:500, IHC 1:50

预测分子量

88.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Phosphodiesterases (PDEs) are a family of related phosphohydrolyases that selectively catalyze the hydrolysis of 3'cyclic phosphate bonds in adenosine and/or guanine 3',5' cyclic monophosphate (cAMP and/or cGMP). They regulate the cellular levels, localization and duration of action of these second messengers by controlling the rate of their degradation. There are 11 subtypes of PDEs, named PDE1-11; PDE4, 7 and 8 selectively degrade cAMP, PDE5, 6 and 9 selectively degrade cGMP and PDE1, 2, 3, 10 and 11 degrade both cyclic nucleotides. PDEs are expressed ubiquitously,with each subtype having a specific tissue distribution. These enzymes are involved in many signal transduction pathways and their functions include vascular smooth muscle proliferation and contraction, cardiac contractility,platelet aggregation, hormone secretion, immune cell activation, and they are involved in learning and memory.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PDE10A monoclonal antibody (1:200).

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of thyroid tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Ovary tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded bladder tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3095, Dilution 1:50)

HEK293T cells were transfected with the pCMV6-ENTRY control (Lane 1), human PDE10A transcript variant 2 (Lane 2) , human PDE10A transcript variant 1 (Lane 3), and mouse PDE10A (Lane 4) cDNA clones for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A.