BD-PE3069-HSPA8 (11X4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HSPA8 (11X4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3069

产品名称

HSPA8 (11X4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

HSPA8

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HSPA8(NP_006588) produced in HEK293T cell.

稀释度

WB 1:1000, IHC 1:150, IF 1:100

预测分子量

70.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG3

背景介绍

This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HSPA8 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, BD-PE3069)

Anti-HSPA8 mouse monoclonal antibody (BD-PE3069) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA8 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA8(Cat# BD-PE3069). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.