BD-PE3059-IGF2BP2 (14X13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IGF2BP2 (14X13) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE3059

产品名称

IGF2BP2 (14X13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IGF2BP2

推荐应用

反应种属

Human,Dog,Monkey,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IGF2BP2 (NP_006333) produced in HEK293T cell.

稀释度

WB 1:1000~2000, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

65.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3059, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3059, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-IGF2BP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE3059, Dilution 1:50)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-IGF2BP2 antibody (BD-PE3059), and then analyzed by flow cytometry.

Anti-IGF2BP2 mouse monoclonal antibody (BD-PE3059) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.