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PDLIM5 (15L14) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE3028
产品名称
PDLIM5 (15L14) Mouse Monoclonal antibody
类别
常规抗体
基因名称
PDLIM5
推荐应用
WB
反应种属
Human,Mouse,Rat
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human PDLIM5(NP_006448) produced in HEK293T cell.
稀释度
WB 1:2000, FCM 1:100
预测分子量
63.8kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2a
背景介绍
The protein encoded by this gene is a LIM domain protein. LIM domains are cysteine-rich double zinc fingers composed of 50 to 60 amino acids that are involved in protein-protein interactions. LIM domain-containing proteins are scaffolds for the formation of multiprotein complexes. The proteins are involved in cytoskeleton organization, cell lineage specification, organ development, and oncogenesis. The encoded protein is also a member of the Enigma class of proteins, a family of proteins that possess a 100-amino acid PDZ domain in the N terminus and 1 to 3 LIM domains in the C terminus. Multiple transcript variants encoding different isoforms have been found for this gene, although not all of them have been fully characterized. [provided by RefSeq]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM5 antibody (BD-PE3028), and then analyzed by flow cytometry.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM5. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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