OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PROCR mouse monoclonal antibody (BD-PE3011). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3011) (1:200)

Immunohistochemical staining of paraffin-embedded human normal adjacent endometrium using anti-PROCR mouse monoclonal antibody (BD-PE3011) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is very strong in the vascular endothelia cells and weak nuclear and cytoplasmic staining in in the glandular endometrial cells.

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3011) (1:200)

Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-PROCR mouse monoclonal antibody (BD-PE3011) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is very strong in the vascular endothelia cells and weak nuclear staining in ovarian tumor cells.

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3011) (1:200)

Immunohistochemical staining of paraffin-embedded human brain using anti-PROCR mouse monoclonal antibody (BD-PE3011) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the molecular layer.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7).

Western blot analysis of extracts (25ug) from 4 different cell lines by using anti-PROCR monoclonal antibody. (1:500) (1: HEK293; 2: Hela; 3: MCF7; 4: HepG2) Dilution: 1:500

Immunohistochemical staining of 2 cases of paraffin-embedded human placenta using anti-PROCR mouse monoclonal antibody (BD-PE3011) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong membraneous staining in the trophoblast cells. The endothelial cells show both cytoplasmic and membraneous staining.

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3011) (1:200)

Immunohistochemical staining of paraffin-embedded human thyroid cancer using anti-PROCR mouse monoclonal antibody (BD-PE3011) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Cytoplasmic staining is very strong in the vascular endothelia cells no staining in tumor cells.

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PROCR mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min, BD-PE3011) (1:200)