BD-PE2981-SMC1A (15T12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SMC1A (15T12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2981

产品名称

SMC1A (15T12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SMC1A

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 889-1016 of human SMC1A (NP_006297) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:1000

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1B or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. Mutations in this gene result in Cornelia de Lange syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 7 different cell lines by using anti-SMC1A monoclonal antibody (1:500).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SMC1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2981) (1:1000)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMC1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMC1A (1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.