BD-PE2974-XRCC1 (14A4) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

XRCC1 (14A4) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2974

产品名称

XRCC1 (14A4) Mouse Monoclonal antibody

类别

常规抗体

基因名称

XRCC1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.

稀释度

WB 1:2000, FCM 1:100

预测分子量

69.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of Jurkat cells, using anti-XRCC1 antibody (BD-PE2974), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-XRCC1 monoclonal antibody (1:200).

Flow cytometric Analysis of Hela cells, using anti-XRCC1 antibody (BD-PE2974), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1 (1:1000).