BD-PE2889-IRF6 (5S6) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IRF6 (5S6) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2889

产品名称

IRF6 (5S6) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IRF6

推荐应用

反应种属

Human,Monkey,Dog,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IRF6(NP_006138) produced in HEK293 Cell.

稀释度

WB 1:500, IHC 1:100

预测分子量

52.9kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded human tonsil composit using anti-IRF6 06 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE2889); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in both the 20 and 40x however the 20x reveal the the squamous epithelial are positive for IRF6 and the cells of the germinal and non germinal centers are negative for IRF6.

Immunohistochemical staining of paraffin-embedded mouse testis using mouse anti-IRF6 06 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE2889 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500

Immunohistochemical staining of paraffin-embedded mouse epididymis tissue using mouse anti-IRF6 06 mouse monoclonal antibody. HIER TEE buffer (pH9) at 110C for 10 min, BD-PE2889 (1:100). Detection was done with Klear Mouse (C/N ) DAB Chromogen Kit.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-IRF6 mouse monoclonal antibody. (BD-PE2889; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-IRF6 mouse monoclonal antibody. (BD-PE2889; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded human endometrium using anti-IRF6 06 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE2889); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in placenta.

Immunofluorescent staining of HeLa cells using anti-IRF6 mouse monoclonal antibody (BD-PE2889, green, 1:100). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human placenta using anti-IRF6 06 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE2889); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong nuclear staining is seen in placenta.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IRF6 mouse monoclonal antibody. (BD-PE2889; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)