BD-PE2814-S100P (19H5) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

S100P (19H5) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2814

产品名称

S100P (19H5) Mouse Monoclonal antibody

类别

常规抗体

基因名称

S100P

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human S100P(NP_005971) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:1000

预测分子量

10.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-S100P mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-S100P very specifically recognizes S100P antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded human liver cancer using anti-S100P mouse monoclonal antibody (BD-PE2814) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear, cytoplasmic, and membrane staining is seen in infiltrating lymphacytes and no staining is tumor cells.

Immunohistochemical staining of paraffin-embedded human spleen using anti-S100P mouse monoclonal antibody (BD-PE2814) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear and cytoplasmic staining is seen in red pulp cells.

Immunohistochemical staining of paraffin-embedded human gastric cancer using anti-S100P mouse monoclonal antibody (BD-PE2814) at 1:200 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Nuclear and cytoplasmic staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded human placenta using anti-S100P mouse monoclonal antibody @ 2 ug/mL with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with Citrate pH6.1

Immunofluorescent staining of HeLa cells using anti-S100P mouse monoclonal antibody (BD-PE2814, green, 1:50). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded human colon cancer using anti-S100P mouse monoclonal antibody @ 2 ug/mL with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with Citrate pH6.0

Immunohistochemical staining of paraffin-embedded mouse small intestine tissue using anti-S100P mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2814 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-S100Pmouse monoclonal antibody.