BD-PE2785-SMAD2 (11L8) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SMAD2 (11L8) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2785

产品名称

SMAD2 (11L8) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SMAD2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SMAD2 (NP_005892) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM: 1:100

预测分子量

52.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric analysis of living 293T cells transfected with SMAD2 overexpression plasmid , Red)/empty vector (Blue) using anti-SMAD2 antibody (BD-PE2785). Cells incubated with a non-specific antibody (Green) were used as isotype control (1:100).

Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY SMAD2 using anti-SMAD2 antibody (BD-PE2785/green, left). 293T cells transfected with empty vector served as a negative control (right) (1:100).

Flow cytometric Analysis of permeabilized HUVEC cells, using anti-SMAD2 antibody (BD-PE2785), (Red), compared to an IgG isotype control, (green) (1:100).

Flow cytometric Analysis of Jurkat cells, using anti-SMAD2 antibody (BD-PE2785), (Red), compared to a nonspecific negative control antibody, (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMAD2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SMAD2(Cat# BD-PE2785). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.