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CALCOCO2 (14R16) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE2761
产品名称
CALCOCO2 (14R16) Mouse Monoclonal antibody
类别
常规抗体
基因名称
CALCOCO2
推荐应用
WB
反应种属
Human,Monkey
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
稀释度
WB 1:500~2000, IF 1:100, FCM 1:100
预测分子量
52.1kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (BD-PE2761), and then analyzed by flow cytometry.

Flow cytometric Analysis of Jurkat cells, using anti-CALCOCO2 antibody (BD-PE2761), (Red), compared to a nonspecific negative control antibody, (Blue).

Figure from citation: Western Blot of CALCOCO2 (NDP52) protein level by using anti-CALCOCO2 antibody in human HEp-2 cell lysates.

Flow cytometric Analysis of Hela cells, using anti-CALCOCO2 antibody (BD-PE2761), (Red), compared to a nonspecific negative control antibody, (Blue).

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-CALCOCO2 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CALCOCO2 monoclonal antibody.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2(Cat# BD-PE2761). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

Anti-CALCOCO2 mouse monoclonal antibody (BD-PE2761) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CALCOCO2 .

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