BD-PE2723-SSX1 (15Z3) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SSX1 (15Z3) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2723

产品名称

SSX1 (15Z3) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SSX1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SSX1 (NP_005626) produced in E.coli.

稀释度

WB 1:500~2000, IHC 1:150, IF 1:100, FCM 1:100

预测分子量

21.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSX1 antibody (BD-PE2723), and then analyzed by flow cytometry.

Flow cytometric Analysis of Hela cells, using anti-SSX1 antibody (BD-PE2723), (Red), compared to a nonspecific negative control antibody, (Blue).

Flow cytometric Analysis of Jurkat cells, using anti-SSX1 antibody (BD-PE2723), (Red), compared to a nonspecific negative control antibody, (Blue).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2723)

Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2723)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2723)

Anti-SSX1 mouse monoclonal antibody (BD-PE2723) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX1 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.