BD-PE2721-SDCBP (17Z12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

SDCBP (17Z12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2721

产品名称

SDCBP (17Z12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

SDCBP

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human SDCBP(NP_005616) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500, IHC 1:100

预测分子量

32.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-SDCBP mouse monoclonal antibody (BD-PE2721). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.

Immunohistochemical staining of paraffin-embedded composit of 2 human melanoma cases using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane, nuclear, and cytoplasmic staining is seen in tumor cells with various intensity.

Immunohistochemical staining of paraffin-embedded tonsil using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in germinal and non germinal center of the the tonsil.

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-SDCBP (SYNTINEN) 9 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2721 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded human brain using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Weak membrane and cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded composit of 3 human endometrial cancer cases using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

Immunohistochemical staining of paraffin-embedded composit of 3 human liver cancer cases using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

Immunohistochemical staining of paraffin-embedded composit of 2 human spleen cases using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in the cells of red pulp and white pulp.

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SDCBP monoclonal antibody .

Immunohistochemical staining of paraffin-embedded composit of 3 human ovarian cancer cases using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells with various intensity.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SDCBP mouse monoclonal antibody. (BD-PE2721; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)

Immunohistochemical staining of paraffin-embedded mouse lung tissue using anti-SDCBP (SYNTINEN) 9 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2721 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-SDCBP 9 mouse monoclonal antibody (BD-PE2721) at 1:100 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Citrate pH6.0 HIER buffer using pressure chamber for 3 minutes at 110C. Membrane and cytoplasmic staining is seen in tumor cells.

Immunohistochemical staining of paraffin-embedded mouse kidney tissue using anti-SDCBP (SYNTINEN) 9 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2721 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded mouse spleen tissue using anti-SDCBP (SYNTINEN) 9 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2721 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SDCBP monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Immunofluorescent staining of MCF-7 cells using anti-SDCBP mouse monoclonal antibody (BD-PE2721, green, 1:50). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue).

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-SDCBP mouse monoclonal antibody. (BD-PE2721; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min)