BD-PE2677-IDH3A (2U14) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

IDH3A (2U14) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2677

产品名称

IDH3A (2U14) Mouse Monoclonal antibody

类别

常规抗体

基因名称

IDH3A

推荐应用

反应种属

Human,Mouse,Rat,Dog

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human IDH3A (NP_005521) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100, FCM 1:100

预测分子量

39.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-IDH3A monoclonal antibody.

Figure from citation: Western blot analysis of IDH3A protein level by using anti-IDH3A antibody in fibroblasts with miR-424 knockdown or overexpression, and in TGF-b1-treated fibroblasts with or without miR-424 depletion.

Flow cytometric analysis of Hela cells, using anti-IDH3A antibody (BD-PE2677), (Red) compared to a nonspecific negative control antibody (Blue).

Immunofluorescent staining of HepG2 cells using anti-IDH3A mouse monoclonal antibody (BD-PE2677).

Anti-IDH3A mouse monoclonal antibody (BD-PE2677) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY IDH3A .

Flow cytometric analysis of Jurkat cells, using anti-IDH3A antibody (BD-PE2677), (Red) compared to a nonspecific negative control antibody (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IDH3A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IDH3A(Cat# BD-PE2677). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.