OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-Melan A (MLANA) mouse monoclonal antibody (BD-PE2668). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. (1:100)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Melan A (MLANA) (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Melan A (MLANA). (1:1000)

Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-Melan A (MLANA) mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120 oC for 3 min, BD-PE2668) (1:1600)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HNF1-Beta (HNF1B) rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH9.0) at 120°C for 3 min, RM1396) (1:500)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-SOX11 rabbit monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer {pH8.0} at 120°C for 3 min, RM1397)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX11 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX11. (Cat# RM1397)(1:10000)