BD-PE2658-BCAT1 (4M19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

BCAT1 (4M19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2658

产品名称

BCAT1 (4M19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

BCAT1

推荐应用

反应种属

Human,Dog,Mouse

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human BCAT1(NP_005495) produced in HEK293T cell.

稀释度

WB 1:500~2000, IHC 1:150

预测分子量

42.8kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-BCAT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2658)

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon).

Western blot analysis of extracts (10ug) from a mouse cell line and a mouse tissue by using anti-BCAT1 monoclonal antibody (1:200).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCAT1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCAT1(Cat# BD-PE2658). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.