BD-PE2647-AKT3 (7P12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AKT3 (7P12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2647

产品名称

AKT3 (7P12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

AKT3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 2-479 of human AKT3

稀释度

WB: 1:500, IF (1:100)

预测分子量

55.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG3

背景介绍

AKT3 is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1)

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunofluorescent staining of HeLa cells using anti-AKT3 mouse monoclonal antibody (BD-PE2647).

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-AKT3 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-AKT3 mouse monoclonal antibody (BD-PE2647) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY AKT3

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.