BD-PE2646-AKT3 (13L13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

AKT3 (13L13) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE2646

产品名称

AKT3 (13L13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

AKT3

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human AKT3 (NP_005456) produced in E.coli.

稀释度

WB 1:500, IHC 1:50, IF 1:100, FCM 1:100

预测分子量

55.6kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG3

背景介绍

The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-AKT3 monoclonal antibody at 1:200 dilution.

Figure from citation: Western Blot of Akt3 protein level by using anti-Akt3 antibody in rat endometrium during pregnancy and pseudopregnancy.

AKT3 antibody at 1:500 dilution + BALB/C 3T3 cell lysate

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AKT3 antibody (BD-PE2646), and then analyzed by flow cytometry.

Anti-AKT3 mouse monoclonal antibody (BD-PE2646) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT3 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AKT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AKT3 (Cat# BD-PE2646).

Immunohistochemical staining of paraffin-embedded Human lung tissue within the normal limits using anti-AKT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2646)