BD-PE2640-MAPK8IP1 (13V12) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

MAPK8IP1 (13V12) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2640

产品名称

MAPK8IP1 (13V12) Mouse Monoclonal antibody

类别

常规抗体

基因名称

MAPK8IP1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human MAPK8IP1 (NP_005447) produced in HEK293T cell.

稀释度

IHC 1:150

预测分子量

77.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a regulator of the pancreatic beta-cell function. It is highly similar to JIP-1, a mouse protein known to be a regulator of c-Jun amino-terminal kinase (Mapk8). This protein has been shown to prevent MAPK8 mediated activation of transcription factors, and to decrease IL-1 beta and MAP kinase kinase 1 (MEKK1) induced apoptosis in pancreatic beta cells. This protein also functions as a DNA-binding transactivator of the glucose transporter GLUT2. RE1-silencing transcription factor (REST) is reported to repress the expression of this gene in insulin-secreting beta cells. This gene is found to be mutated in a type 2 diabetes family, and thus is thought to be a susceptibility gene for type 2 diabetes. [provided by RefSeq, May 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MAPK8IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2640) (1:150)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue tissue using anti-MAPK8IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2640) (1:150)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MAPK8IP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2640) (1:150)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MAPK8IP1 mouse monoclonal antibody. This figure shows negative staining. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2640) (1:150)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAPK8IP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAPK8IP1 (Cat# BD-PE2640)(1:2000). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.