BD-PE2577-GRB10 (11F13) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

GRB10 (11F13) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2577

产品名称

GRB10 (11F13) Mouse Monoclonal antibody

类别

常规抗体

基因名称

GRB10

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 164-415 of human GRB10 (NP_005302) produced in E.coli.

稀释度

WB 1:500, IHC 1:500

预测分子量

67.1kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GRB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2577) (1:500)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-GRB10 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2577) (1:500)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRB10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRB10(Cat# BD-PE2577). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.