Immunohistochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pCINEO-hGPR17 or pCINEO plasmids using anti-GPR17 Mouse monoclonal antibody (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min) (1:150).

Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2573) (1:600)

Immunohistochemical staining of paraffin-embedded mouse cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2573) (1:200)

Western blot analysis of lysates from 293T cells transfected with (1)pcDNA3.1-mGPR17, (2)pcDNA3.1-rGPR17, (3)pCINEO-hGPR17, (4)pcDNA3.1, (5)PCINEO plasmids using anti-GPR17 Mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded rat cerebrum tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2573) (1:200)

Immunohistochemical staining of paraffin-embedded Human adult brain tissue within the normal limits using anti-GPR17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, BD-PE2573) (1:600)

Immunohistochemical staining of paraffin-embedded cell pellets of 293T cells transfected with pcDNA3.1-mGPR17, pcDNA3.1-rGPR17 or pcDNA3.1 plasmids using anti-GPR17 Mouse monoclonal antibody (Heat-induced epitope retrieval by Tris-EDTA buffer (pH8.0) at 120°C for 2.5 min) (1:150).