BD-PE2558-FOS (15V1) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

FOS (15V1) Mouse Monoclonal antibody

产品详情相关文献产品问答相关产品

产品基本信息

产品货号

BD-PE2558

产品名称

FOS (15V1) Mouse Monoclonal antibody

类别

常规抗体

基因名称

FOS

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human FOS (NP_005243) produced in E.coli.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~200

预测分子量

40.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-FOS mouse monoclonal antibody (BD-PE2558). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-FOS mouse monoclonal antibody.HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE2558 was diluted 1:200 and detection was done with HRP secondary and DAB chromogen. Nuclear staining seen in tumor cells.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-FOS at 1:200 dilution of 0.5 mg/mL and detection with Polink2 Broad HRP DAB. BD-PE2558 requires heat-induced epitope retrieval with ACCEL (pH8.7) biocare pressure cooker. The image shows nuclear staining in tumor cells.

Immunohistochemical staining of paraffin-embedded carcinoma of human thyroid tissue using anti-FOS mouse monoclonal antibody.HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE2558 was diluted 1:200 and detection was done with HRP secondary and DAB chromogen. Nuclear staining seen in tumor cells.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FOS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOS (1:4000).

Immunohistochemical staining of paraffin-embedded human bladder tissue within the normal limits using anti-FOS mouse monoclonal antibody.HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE2558 was diluted 1:200 and detection was done with HRP secondary and DAB chromogen. Nuclear staining seen in epithelial cells.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FOS mouse monoclonal antibody.HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE2558 was diluted 1:200 and detection was done with HRP secondary and DAB chromogen. Nuclear staining seen in tumor cells.

Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-FOS mouse monoclonal antibody. HIER pretreatment was done with 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 minutes. BD-PE2558 was diluted 1:200 and detection was done with HRP secondary and DAB chromogen. Here we see nuclear staining.