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CD80 (19A10) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE2525
产品名称
CD80 (19A10) Mouse Monoclonal antibody
类别
常规抗体
基因名称
CD80
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human CD80 (NP_005182) produced in HEK293T cell.
稀释度
WB 1:500, FCM 1:100
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG1
背景介绍
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

CD80 Luminex with 7D6 (BD-PE2525) Capture and 11G5 Detection Antibodies. Substrate used: Recombinant Human CD80

Flow cytometric Analysis of living Raji cells, using anti-CD80 antibody (BD-PE2525), (Red), compared to an IgG isotype control, (Blue) (1:100).

HEK293T cells transfected with either overexpress plasmid (Red) or IgG isotype control (Blue) were immunostained by anti-CD80 antibody (BD-PE2525), and then analyzed by flow cytometry (1:100).

CD80 ELISA with 7D6 (BD-PE2525) Capture and 11G5 Detection Antibodies. Substrate used: Recombinant Human CD80

Flow cytometric Analysis of living Jurkat cells, using anti-CD80 antibody (BD-PE2525), (Red), compared to an IgG isotype control, (Blue) (1:100).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD80 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD80 (1:500).

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