BD-PE2505-HP (5G10) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HP (5G10) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2505

产品名称

HP (5G10) Mouse Monoclonal antibody

类别

常规抗体

基因名称

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HP (NP_005134) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

WB 1:500~2000, IF 1:100

预测分子量

43.3kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a preproprotein, which is processed to yield both alpha and beta chains, which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin, which allows degradative enzymes to gain access to the hemoglobin, while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy, the incidence of coronary artery disease in type 1 diabetes, Crohn's disease, inflammatory disease behavior, primary sclerosing cholangitis, susceptibility to idiopathic Parkinson's disease, and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-HPmouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded breast tissue using anti-HP mouse monoclonal antibody.

Immunofluorescent staining of HepG2 cells using anti-HP mouse monoclonal antibody (BD-PE2505, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.

Immunohistochemical staining of paraffin-embedded lung tissue using anti-HPmouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded mouse liver tissue using anti-HP 0 mouse monoclonal antibody. HIER TEE buffer pH9 at 110C for 10 min, BD-PE2505 (1:100). Detection was done with Klear Mouse (C/N ) DAB Kit.

Immunohistochemical staining of paraffin-embedded pancreas tissue using anti-HPmouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded prostate tissue using anti-HPmouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded human placenta using anti-HP 0 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE2505); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong membraneous staining is seen in placenta and adjacent blood.

Immunohistochemical staining of paraffin-embedded human gastric cancer seen mainly normal adjacent using anti-HP 0 mouse monoclonal antibody at 1:200 with Polink2 Broad HRP DAB detection kit (BD-PE2505); heat-induced epitope retrieval with citrate buffer, pH6.0at 95-100C. Strong cytoplasmic and membraneous staining is seen in gastric glands.

Immunohistochemical staining of paraffin-embedded human ovarian cancer using anti-HP 0 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE2505 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tumor cells shows membrane and cytoplasmic staining.

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-HP mouse monoclonal antibody.

Immunohistochemical staining of paraffin-embedded human liver cancer using anti-HP 0 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE2505 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. The tumor cells shows membrane and cytoplasmic staining.

Western Blot analysis of 10 different human tissue lysates (10ug) by using anti-HP monoclonal antibody

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HP monoclonal antibody at 1:500.

Immunohistochemical staining of paraffin-embedded Carcinoma of pancreas tissue using anti-HPmouse monoclonal antibody.

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-HP mouse monoclonal antibody . The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification. These data show that UltraMAB anti-HP very specifically recognizes HP antigen on OriGene protein microarray chip.

Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-HPmouse monoclonal antibody.