OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-TLE1 mouse monoclonal antibody (BD-PE2486). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Immunohistochemical staining of paraffin-embedded cell pellets (Sample1 is A-431, sample2 is NCI-H2228, sample3 is MDA-MB-453 and sample4 is HCT-15) using anti-TLE1 Mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human cervix uterus within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human neurilemmoma tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TLE1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TLE1 (1:2000).

Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human synovial sarcoma tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunofluorescent staining of 4 different cell lines (MCF7, NCI-H2228, A431, HCT-15) using anti-TLE1 mouse monoclonal antibody (BD-PE2486) (1:100).

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-TLE1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 2.5 min, BD-PE2486) (1:300)