BD-PE2459-PD-1 (PDCD1) (9G11) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PD-1 (PDCD1) (9G11) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2459

产品名称

PD-1 (PDCD1) (9G11) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PDCD1

推荐应用

反应种属

Human,Mouse,Monkey,Dog

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.

特异性

Validated through 10k CHIP testing，using a High-Density Protein Microarray that was spotted with ~17,000 various over-expressed proteins to test for antibody specificity.

稀释度

IHC 1:100~1200, WB 1:500, IF 1:100

预测分子量

29.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-PDCD1 mouse monoclonal antibody (BD-PE2459). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification (1:100).

Western blot analysis of extracts (35ug) from PBMCs and PHA-stimulated-PBMCs by using anti-PDCD1 monoclonal antibody (1:250).

Immunohistochemical staining of paraffin-embedded human lung cancer using anti-PD-1 98 mouse monoclonal antibody (BD-PE2459) at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-PD-1 98 mouse monoclonal antibody (BD-PE2459) at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells no staining on the tumor.

Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:500.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1 antibody (1:2000).

Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Thyroid, 7: Colon, 8: Spleen 9: Liver, 10: Ovary). Diluation: 1:500.

Immunocytochemistry staining of PDCD1 -stable-expression cells (left) labeling PDCD1 with mouse anti-PDCD1 monoclonal antibody BD-PE2459 (1:900). The rihgt is negative control.

Immunohistochemical staining of 3 cases of paraffin-embedded human tonsil using anti-PD-1 98 mouse monoclonal antibody (BD-PE2459) at 1:800 with Polink2 Broad HRP DAB detection kit; heat-induced epitope retrieval with GBI Accel pH8.7 HIER buffer using pressure chamber for 3 minutes at 110C. Very strong cytoplasmic and membraneous staining in the activated Tcells of tonsil.

Immunofluorescent staining of PDCD1 -stable-expression cells (left) labeling PDCD1 with mouse monoclonal antibody BD-PE2459 (1:100, green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right).

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 110°C for 10min, BD-PE2459) (1:1200)