BD-PE2453-PD-1 (PDCD1) (5M9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

PD-1 (PDCD1) (5M9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2453

产品名称

PD-1 (PDCD1) (5M9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

PDCD1

推荐应用

反应种属

Human

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human PDCD1 (NP_005009) produced in HEK293T cell.

稀释度

WB 1:2000, IF 1:100~900, FCM 1:50

预测分子量

29.2kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

PD1 Luminex ELISA with 1B11 Capture and 17G9 Detection Antibodies. Substrate used: Recombinant Human PD1

Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody (BD-PE2453) (Red) compared to a nonspecific negative control antibody (Blue) (1:50).

PD1 Luminex ELISA with 7B4 Capture and 17G9 Detection Antibodies. Substrate used: Recombinant Human PD1

Immunocytochemistry staining of stable expression PD1 cells using anti-PDCD1 mouse monoclonal antibody (BD-PE2453) (Left). The rihgt is negative control. (1:100) (1:900)

Flow cytometric Analysis of stable expression PDL1 cells using anti-PDCD1 antibody (BD-PE2453) (blue) or 0.3ug/ml PD1-Fc fusion protein (green) or both (red), and detected by anti-Fc (human) IgG-FITC (1:50).

Immunofluorescent staining of PDCD1 -stable-transfected HEK293T cells (left) labeling PDCD1 with mouse monoclonal antibody BD-PE2453 (green) and nucleus with Hoechst33342 (blue). HEK293T cells serve as negative control (right). n (1:100)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDCD1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDCD1. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.