Western blot analysis of extracts (35ug) from PBMCs and PHA-stimulated-PBMCs by using anti-PDCD1 monoclonal antibody (1:250).

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-PDCD1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, BD-PE2447)

Flow cytometric Analysis of stable expression PD1 cells using anti-PDCD1 antibody (BD-PE2447) (Red) compared to a nonspecific negative control antibody (Blue). (1:100) (1:50)