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NME4 (2S8) Mouse Monoclonal antibody

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产品基本信息

产品货号
BD-PE2443
产品名称
NME4 (2S8) Mouse Monoclonal antibody
类别
常规抗体
基因名称
NME4
推荐应用
WB
反应种属
Human
存储缓冲液
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.
免疫原
Full length human recombinant protein of human NME4 (NP_005000) produced in HEK293T cell.
稀释度
WB 1:2000, IHC 1:50, IF 1:100
预测分子量
20.6kDa
运输及保存条件
-20°C/1 year
宿主
Mouse
同种型
IgG2b
背景介绍
The nucleoside diphosphate (NDP) kinases (EC 2.7.4.6) are ubiquitous enzymes that catalyze transfer of gamma-phosphates, via a phosphohistidine intermediate, between nucleoside and dioxynucleoside tri- and diphosphates.The enzymes are products of the nm23 gene family, which includes NME4.
期货
现货
纯化
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NME4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2443, Dilution 1:50)

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-NME4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2443, Dilution 1:50)

Anti-NME4 mouse monoclonal antibody (BD-PE2443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4 .

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME4. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.

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