BD-PE2424-HNRNPF (hnRNP F) (11C19) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HNRNPF (hnRNP F) (11C19) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2424

产品名称

HNRNPF (hnRNP F) (11C19) Mouse Monoclonal antibody

类别

常规抗体

基因名称

HNRNPF

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HNRNPF (NP_004957) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:50, IF 1:50~100, FCM 1:100

预测分子量

45.7kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2424)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2424)

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2424)

Flow cytometric Analysis of Hela cells, using anti-HNRNPF antibody (BD-PE2424), (Red), compared to a nonspecific negative control antibody , (Blue).

Immunofluorescent staining of HepG2 cells using anti-HNRNPF mouse monoclonal antibody (BD-PE2424).

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2424)

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HNRNPF mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2424)

Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HNRNPF monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

Anti-HNRNPF mouse monoclonal antibody (BD-PE2424) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HNRNPF .

Flow cytometric Analysis of Jurkat cells, using anti-HNRNPF antibody (BD-PE2424), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HNRNPF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HNRNPF(Cat# BD-PE2424). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.