BD-PE2416-DOCK2 (12V7) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

DOCK2 (12V7) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2416

产品名称

DOCK2 (12V7) Mouse Monoclonal antibody

类别

常规抗体

基因名称

DOCK2

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Human recombinant protein fragment corresponding to amino acids 1544-1830 of human DOCK2 (NP_004937) produced in E.coli.

稀释度

WB 1:2000, IHC 1:150

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2a

背景介绍

The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells, predominantly in the peripheral blood leukocytes, and is involved in remodeling of the actin cytoskeleton required for lymphocyte migration, through the activation of RAC. Mice lacking this gene show a severe impairment in the migration and homing of lymphocytes. These mutant mice also exhibited long-term survival of allografts, suggesting that this gene may be a target for controlling transplant rejection. [provided by RefSeq, Oct 2011]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded human tonsil using anti-DOCK2 42 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE2416 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. IHC staining shows germinal and nongerminal center with strong stain in the cytoplasm and membrane.

Immunofluorescent staining of Jurkat cells using anti-DOCK2 mouse monoclonal antibody (BD-PE2416, green, 1:100). Actin filaments were labeled with Alexa Fluor® 594 Phalloidin (red), and nuclear with DAPI (blue). Scale bar, 20µm.

Western blot analysis of Jurkat cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded human melanoma using anti-DOCK2 42 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE2416 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.

Immunohistochemical staining of paraffin-embedded human lung cancer using anti-DOCK2 42 mouse monoclonal antibody at 1:200 dilution of 1.0 mg/mL using Polink2 Broad HRP DAB for detection. BD-PE2416 requires HIER with with citrate pH6.0 at 110°C for 3min using pressure chamber/cooker. IHC staining shows tumor cells are negative however infiltrating lymphocytes strongly positive.

Western blot analysis of RPMI8226 cell lysate (35ug) by using anti-DOCK2 monoclonal antibody.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-DOCK2 mouse monoclonal antibody. (BD-PE2416; heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)