BD-PE2410-CDKN2B (p15 INK4b) (18N16) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

CDKN2B (p15 INK4b) (18N16) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2410

产品名称

CDKN2B (p15 INK4b) (18N16) Mouse Monoclonal antibody

类别

常规抗体

基因名称

CDKN2B

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human CDKN2B(NP_004927) produced in HEK293T cell.

稀释度

WB 1:2000, IHC 1:150, FCM 1:100

预测分子量

14.5kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG1

背景介绍

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2410)

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CDKN2B antibody (BD-PE2410), and then analyzed by flow cytometry.

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-CDKN2B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, BD-PE2410)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDKN2B. Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.