BD-PE2379-HAND1 (12W9) Mouse Monoclonal antibody-现货抗体产品库武汉枢密脑科学技术有限公司

HAND1 (12W9) Mouse Monoclonal antibody

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产品基本信息

产品货号

BD-PE2379

产品名称

HAND1 (12W9) Mouse Monoclonal antibody

类别

常规抗体

基因名称

HAND1

推荐应用

反应种属

Human,Mouse,Rat

存储缓冲液

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% New type preservative N.

免疫原

Full length human recombinant protein of human HAND1 (NP_004812) produced in E.coli.

稀释度

WB 1:500, IF 1:100, FCM 1:100

预测分子量

23.4kDa

运输及保存条件

-20°C/1 year

宿主

Mouse

同种型

IgG2b

背景介绍

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq]

期货

现货

纯化

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Flow cytometric Analysis of Hela cells, using anti-HAND1 antibody (BD-PE2379), (Red), compared to a nonspecific negative control antibody , (Blue).

Anti-HAND1 mouse monoclonal antibody (BD-PE2379) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1 .

Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-HAND1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Flow cytometric Analysis of Jurkat cells, using anti-HAND1 antibody (BD-PE2379), (Red), compared to a nonspecific negative control antibody , (Blue).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1(Cat# BD-PE2379). Positive lysates (100ug) and (20ug) can be purchased separately from OriGene.