产品名称
Rho A (4E12) Mouse Monoclonal Antibody
别名
Transforming protein RhoA (Rho cDNA clone 12) (h12)
基因名称
RHOA ARH12 ARHA RHO12
存储缓冲液
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.50% New type preservative N.
Human Gene Link
https://www.uniprot.org/uniprot/387
Human Swissprot No.
P61586
Human Swissprot Link
https://www.uniprot.org/uniprotkb/P61586/entry
Mouse Gene Link
https://www.uniprot.org/uniprot/11848
Mouse Swissprot No.
Q9QUI0
Mouse Swissprot Link
https://www.uniprot.org/uniprotkb/Q9QUI0
Rat Gene Link
https://www.uniprot.org/uniprot/117273
Rat Swissprot Link
https://www.uniprot.org/uniprotkb/P61589
免疫原
Synthesized peptide derived from human Rho A
特异性
This antibody detects endogenous levels of Rho A at Human, Mouse,Rat
稀释度
IHC-p1:50-200 ,WB 1:1000-2000
背景介绍
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
组织表达
Adipose tissue,Brain,Colon,Mammary cancer,Oesophageal carcinoma,Placenta,Re
细胞定位
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Cytoplasm, cell cortex . Midbody. Cell projection, lamellipodium . Cell projection, dendrite . Nucleus . Localized to cell-cell contacts in calcium-treated keratinocytes (By similarity). Translocates to the equatorial region before furrow formation in a ECT2-dependent manner. Localizes to the equatorial cell cortex (at the site of the presumptive furrow) in early anaphase in an activated form and in a myosin- and actin-independent manner. .
功能
cofactor:Magnesium.,domain:The basic-rich region is essential for yopT recognition and cleavage.,function:Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP.,PTM:Cleaved by yopT protease when the cell is infected by some Yersinia pathogens. This removes the lipid attachment, and leads to its displacement from plasma membrane and to subsequent cytoskeleton cleavage.,PTM:Substrate for botulinum ADP-ribosyltransferase.,similarity:Belongs to the small GTPase superfamily. Rho family.,subunit:Interacts with RGNEF (By similarity). Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation.,
纯化
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
